Products & ReviewLife Sciences
ImProm-II™ Reverse Transcriptase
Promega Corp.A3801, A3802, A3803
Full-Length cDNA Synthesis for the Analysis of Rare or Long Messages
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Good quality, high reproducibility.
Application Area:
Retro transcription of low amount of viral RNA
This product is very easy to use, allows us to obtain good amounts of viral cDNA when the RNA sample is very poor. Good product, good ratio price/quality.
Review Date: 17 Jan 2019 | Promega Corp.
cDNA Synthesis for the Analysis of Rare or Long Messages
ImProm-II™ Reverse Transcriptase enables robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The ImProm-II™ Reverse Transcriptase can be used to reverse transcribe total RNA, poly(A)+ mRNA or synthetic transcript RNA templates.
Advantages
- Full-Length RT-PCR: Reverse transcribe RNA templates up to 8.9kb.
- Microarray-Compatible: Incorporate regular and Cy®3- and Cy®5-modified nucleotides.
- Scalable and Flexible: Reaction volumes of 1–20μl can be used in subsequent PCR, and the optimized buffer also allows for coupled RT-PCR.
- Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl and 50mM DTT. A 25mM MgCl2 Solution also is included.
- Versatile: Use with your thermostable polymerase of choice for two-step RT-PCR.
- Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
