Human ON-TARGETplus siRNA Library – Genome
siRNA designed and modified for greater specificity
An arrayed collection of siRNA reagents for RNAi screening, targeting the whole human genome. ON-TARGETplus siRNA is guaranteed to silence, and is modified to reduce off-targets for fewer false positives.
Genome-wide siRNA screening has become an invaluable discovery tool, and siRNA products are more often the choice for high quality, reliable screening libraries. The Human ON-TARGETplus
Human Genome siRNA Library targets all unique human genes (NM Accession numbers) in the NCBI RefSeq database.
The ON-TARGETplus siRNA designs and modifications reduce off-target effects while maintaining high silencing potency for high-confidence screening results.
Off-target effects: Disturbing the silence of RNA interference (RNAi)
Off-target effects occur when an siRNA is processed by the RNA-Induced Silencing Complex (RISC) and down-regulates unintended targets. As these changes in gene expression can lead to measurable phenotypes (such as false positives) it is of great importance to understand the mechanism behind off-targeting so that strategies can be developed to minimize their effects. Download this application note by Horizon Discovery to find out what can decrease off-target effects and therefore allow for better gene silencing.
siRNA screening: Development of hit stratification strategies
In this poster, Horizon Discovery compares two different approaches to hit stratification and validation after an initial screen: 1) a multiple reagent approach where two or more individual siRNAs induce the same phenotype and 2) a chemical modification approach where hit confirmation is achieved using pools of siRNA that contain specificity enhancing modifications.
Reducing off-target effects in RNA interference experiments
In this article, Dr. Christian Nievera, senior product manager at Horizon Discovery, discusses how to minimize non-specific effects of siRNA reagents
