GoTaq® 2-Step RT-qPCR System
GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA inp…
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Great product.
RT-diagnostic
The GoTaq 2-step RT-qPCR System allows us to run RT-PCR in a reliable way, in a good time scale.
Review Date: 20 May 2021 | Promega Corp.
Great results. Repeat customers.
Analyze tissue samples
Using this product is an easy process. Results are reliable and the cost of the test is reasonable.
Review Date: 27 Oct 2019 | Promega Corp.
Excellent extraction product, wouldn't change for another brand.
RNA preparation for QPCR, nano string and RNAseq
Excellent RNA extraction kit. RNA produced is of the highest quality and I have used it for QqPCR, nanostring and precious RNAseq samples. The RIN has always been extremely high on TAPE station analysis.
Review Date: 27 Jun 2019 | Promega Corp.
Cancer Research
GoTaq® 2-Step RT-qPCR System is a kind of product I recommend to any PCR routine laboratory; It made my work easy and I had good results using it.
Review Date: 6 Oct 2015 | Promega Corp.
Real Time PCR
This kit is super easy to use and setup from RNA to qRT-PCR. My only concern was the need of standard RNA which I didn’t have. If they can include it in the kit, it will be fantastic.
Review Date: 15 May 2014 | Promega Corp.
GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method.
The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA input. In the second step, cDNA is quantified using the GoTaq®qPCR Master Mix.
Formulated with a hot-start DNA polymerase, the GoTaq® qPCR Master Mix is optimized for reproducibly specific, sensitive, highly efficient qPCR using the standard or fast mode of a real-time PCR instrument. The GoTaq® qPCR Master Mix contains BRYT™ GREEN dye which can be twice as bright as of SYBR® Green I. The superior performance of these two steps allows researchers to observe earlier quantification cycle values over broader linear ranges.
The GoTaq® 2-Step RT-qPCR System provides a ready-to-use kit for analyzing a wide range of RNA targets by combining the high-activity of GoScript™ Reverse Transcription System with the ultra-bright fluorescence of GoTaq® qPCR Master Mix. The system is robust, requires little optimization, is flexible for a variety of applications, and carries the PCR performance guarantee.
RNA Quantification Using the GoTaq® RT-qPCR Systems
Promega GoTaq® products for RNA quantification offer two dye-based systems for accurate, sensitive, broad-range quantification of RNA targets. This application note demonstrates the successful amplification of 11 differentially expressed mRNA targets using the GoTaq® 1-Step RT-qPCR System. One of these targets was also amplified to demonstrate equivalent performance between the GoTaq® 1-Step and GoTaq® 2-Step RT-qPCR Systems. It was found that reactions with the GoTaq® RT-qPCR Systems have a higher change in fluorescence and earlier Cq values than other RT-qPCR systems.
Purification of Amplifiable Nucleic Acid from Oil Palm and Rice Leaves and Seeds Using the Maxwell® 16 LEV Plant DNA Kit
This poster evaluates a novel cellulose-based paramagnetic particle technology to purify nucleic acid (NA) from Elaeis guineensis (oil palm) and Oryza sativa (rice) plant leaf and seed lysates in an automated format using the Maxwell® 16 System. Plant NA purification provides a variety of unique challenges due to the broad diversity of plants, their extracellular structures, and endogenous compounds (e.g. polysaccharides, phenolics) that can co-purify with NA and inhibit downstream enzymatic assays. Extracted NA were evaluated for yield and amplifiability in qPCR and RT-qPCR, and the data collected supports the broad utility of the novel cellulose-based technology and Maxwell® 16 System for automated plant NA purification.
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