Products & ReviewLife Sciences

GoTaq® 1-Step RT-qPCR System

Promega Corp.A6020

Brighter signal for earlier quantification of low and high copy RNA targets in a convenient 1-Step RT-qPCR format GoTaq® 1-Step RT-qPCR System is a reagent system that gives a brighter signal for earlier quantification of low and high copy RNA targets, using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol in a single tube format. The system incorporates BRYT Green® Fluorescent Dye, a novel dye…

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Brighter signal for earlier quantification of low and high copy RNA targets in a convenient 1-Step RT-qPCR format

GoTaq® 1-Step RT-qPCR System is a reagent system that gives a brighter signal for earlier quantification of low and high copy RNA targets, using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol in a single tube format. The system incorporates BRYT Green® Fluorescent Dye, a novel dye that exhibits enhanced fluorescence when bound to dsDNA, and optimized buffer formulations to improve data accuracy and sensitivity of low expressing targets or targets present at low amounts. GoTaq 1-Step RT-qPCR System is compatible with all real-time PCR instruments in both fast and standard cycling conditions.

The optimized formulations of the GoTaq 1-Step RT-qPCR System components have been engineered to enable simple reaction setup and provide consistently efficient, sensitive, broad range and robust performance in all standard or fast instrument platforms.

The components and protocol allow cDNA synthesis using the GoScript™ Reverse Transcription System and quantification using the GoTaq® qPCR Master Mix. GoTaq 1-Step RT-qPCR System can be used with any real-time instrument capable of detecting SYBR® Green I or FAM™ dye and contains a low level of CXR Reference Dye. A separate tube of CXR Reference Dye is included for use with instruments that require a higher level of reference dye.
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Application NoteLife Sciences

RNA Isolation from 3D Microtissues Using the Promega ReliaPrep™ RNA Tissue Miniprep System

This application note details a protocol to isolate total RNA from as little as 1 microtissue (~ 1000 cells) using the ReliaPrep™ RNA Tissue Miniprep System. Isolation of high quality total RNA from cultured cells is important for various analyses in cell-based drug discovery and toxicity. Analytical applications such as quantitative real time PCR or microarray analysis depend on robust RNA isolation procedures.

16 Dec 2015

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GoTaq® 1-Step RT-qPCR System - Promega Corp. - Life Sciences

GoTaq® 1-Step RT-qPCR System

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