GlykoPrep Technology
The Agilent GlykoPrep (formerly ProZyme) platform streamlines N-glycan sample preparation.
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From purification to glycan release and labeling, the entire sample preparation workflow can be completed in as little as 3 hours. The cartridge-based platform supports traditional glycan labels such as 2-AB and APTS. Innovative high-performance dyes such as InstantPC are also supported. Your lab will enjoy improved efficiency and enhanced precision with GlykoPrep.
- The GlykoPrep platform supports traditional glycan labels such as 2-AB and APTS, as well as the first commercially available instant label for glycans, InstantAB, and the innovative InstantPC.
- GlykoPrep is modular and can be integrated into any workflow. Kit components are also available individually as Digestion Module and dye-specific Labeling & Cleanup Modules, with optional AssayMAP PA50 module for purification of Fc-containing antibodies.
- GlykoPrep is built on AssayMAP technology - microchromatography in a 96-well format. In the manual protocol, GlykoPrep is performed using centrifugation to move liquid through the cartridges (spin format). The workflow is automatable on the Agilent AssayMAP Bravo, see GlykoPrep-plus kit webpages for more detail.
Development of a rapid 2-AB sample preparation workflow for N-glycan release and labeling
In this application note, Agilent Technologies presents a comparison study consisting of two monoclonal antibodies (MabThera, NISTmAb) and one Fc fusion protein (Enbrel), using two different N-glycan sample preparation workflows it offers: 1) Agilent GlykoPrep Rapid N-Glycan Prep with 2-AB; 2) Agilent AdvanceBio Gly-X N-Glycan Prep with 2-AB Express (formerly ProZyme).
N-glycan analysis: Better together
In this brochure, Agilent Technologies introduces the Agilent and ProZyme sample preparation workflows that are designed to help push your glycoprotein analysis to new levels of performance.
