FLUOstar® Omega Plate Reader

This application note by BMG LABTECH describes how the LAMP assay offers an opportunity to work in high throughput increasing diagnostic capacity as an alternative or addition to PCR techniques.

A book of application notes for all assay needs, using BMG LabTech Microplate Readers. This collection showcases the specific capabilities of the different models from the range of BMG LabTech Microplate Readers available.

This application note looks at the quantification of nucleic acids for next-generation sequencing (NGS) applications using the FLUOstar Omega microplate reader. A fluorescent nucleic acid quantification was performed in high-throughput screening format and the results were integrated into the automatic workflow of NGS sample preparation. Here it was shown that combining Qubit assays with the FLUOstar Omega multi-mode microplate reader is ideally suited to measure a wide range of nucleic acids and improves compared to the single tube fluorometer on sensitivity, precision and speed. Especially large laboratories that perform automated high throughput sample preparation will profit from this technology.

The Dual-Luciferase® Reporter Assay or DLR is widely used to study gene transcription and regulation. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla. The Omega series of microplate readers, as well as the CLARIOstar and the PHERAstar FS from BMG LABTECH, have been granted DLReady™ certification based on the results published in this application note.

This application note presents how the Qubit® dsDNA HS fluorophore can be used to quantitate dsDNA on a BMG LABTECH microplate reader.

Discover a novel fluorescence assay that detects interactions of nucleic acids with proteins to identify binding, sequence and structure specificities or dissociation constants. The assay is quick and cost-effective and can be read on a fluorescence reader, such as the FLUOstar® Omega.

Measuring the protein concentration of liquid samples is a routine analysis in many life science laboratories. Accurate quantification is often a critical step for subsequent analyses such as protein characterization or western blots. Spectrometer-based BMG LABTECH readers capture absorbance spectra or absorbance at discrete wavelengths from 220-1000 nm in less than a second per well and therefore can easily read these assays.

One of the most informative ways to increase the biological relevance of an in vitro assay using 3D cell culture is to measure cell metabolism, whereby perturbed metabolism or mitochondrial function is probed without disrupting the 3D structure. This application note describes use of the FLUOstar Omega microplate reader from BMG LABTECH for the sensitive and high throughput measurement of oxygen consumption and extracellular acidification in 3D cell cultures.

This application note demonstrates that a carousel reactor could be used in conjunction with the FLUOstar Omega to conveniently monitor the kinetics of twelve reactions in parallel.

This poster demonstrates that the FLUOstar Omega can be used to measure the GFP fluorescence within living moss protoplasts and that this assay can be used to compare promoter strengths.

Discover tips and considerations for improving microplate reader measurements. Application specialists, Dr. Ann-Cathrin Volz and Dr. Martin Mangold from BMG LABTECH, discuss the impact of different plate reader parameters on data, and provide empirical evidence of their effect on data quality. They also discuss approaches to optimize microplate reader settings, and share recommendations for using BMG LABTECH's range of microplate readers.

Prof. Timo Strünker highlights the flexibility of the BMG LABTECH FLUOstar® and CLARIOstar® microplate readers he uses in his experiments and shares his hopes that this research will help to identify new drugs that could be used as contraceptives. Watch the video to learn more.

This video highlights the Atmospheric Control Unit (ACU), a new accessory for the Omega series of multifunction microplate readers. Two independent intake valves and a microprocessor control panel allow for optimal atmospheric conditions to be maintained inside the microplate reader chamber. The ACU is ideal for creating an environment for all cell based assays, including stem, tumor, mammalian, bacterial, and yeast cells, as well as for mimicking disease states such as hypoxia or anoxia.

Watch this video to see Michael Fejtl from BMG LABTECH presented with the SelectScience Scientists’ Choice Award for Best Drug Discovery Product at ELRIG 2010. This year’s winning product was the FLUOstar Omega Multidetection Microplate Reader, as voted for by SelectScience readers. The award recognises technology which has contributed to drug discovery research.

Watch this video with BMG LABTECH to learn about the new SPECTROstar Nano Microplate Reader. This easy-to-use, push-button reader captures a full UV/Visible spectrum ranging from 220nm to 1000nm in less than 1 second per well. Also find out about the LVis Plate for ultra low volume absorbance measurements. ELRIG 2010 interview.

Find out which products have earned a new Seal of Quality, including the rare Diamond Seal of Quality

Advancing drug discovery and development through cutting-edge innovations

Dr. Jaroslav Fulneček, CEITEC MU, tells SelectScience about a new high-throughput assay for analysis of DNA-protein interactions