Direct Detect® Spectrometer
Introducing Direct Detect™, the first infrared (IR)-based biomolecular quantitation system. Just spot your sample, blank, and read! No sample prep, standard curves, messy cuvettes or liquid waste. With inherent amide bond detection, the Direct Detect™ system distinguishes proteins and peptides from interfering sample components, providing more accurate results without the pitfalls of colorimetric assays. Technology: FTIR Spe…
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Worth for money
Quality Control
Reliable and user friendly equipment, and size small and less space need to install.
Review Date: 6 Jul 2025 | Merck
Introducing Direct Detect™, the first infrared (IR)-based biomolecular quantitation system.
Just spot your sample, blank, and read! No sample prep, standard curves, messy cuvettes or liquid waste. With inherent amide bond detection, the Direct Detect™ system distinguishes proteins and peptides from interfering sample components, providing more accurate results without the pitfalls of colorimetric assays.
Technology: FTIR Spectrometry and Protein Quantitation
By measuring amide bonds in protein chains, the system accurately determines an intrinsic component of every protein without relying on amino acid composition, dye binding or redox potential. Accurately analyze components of complex mixtures: proteins, lipids, carbohydrates, and nucleic acids have separable IR spectra.
Advantages of FTIR Quantitation:
• Independent of amino acid composition
• No extinction coefficient required, unlike UV-Vis/A280 methods
• Improves speed and accuracy over traditional colorimetric assays
• Works in the presence of detergents and reducing agents
• Compatible with lysates and membrane preps
The Direct Detect™ Biomolecular Quantitation System
This application note introduces the Direct Detect™ system which has been optimized for detection and quantitation of proteins. By measuring amide bonds in protein chains, the system accurately determines an intrinsic component of every protein without relying on amino acid composition, dye binding properties or redox potential.
IR-based Protein Quantitation Surpasses Colorimetric Assay Quantitation and Results are Independent of Detergents, Reducing Agents and Analysis Time
Read this application note to learn about a comparison for infrared (IR)-based protein quantitation using the Direct Detect™ quantitation system to Bradford and bicinchoninic acid (BCA) colorimetric assays. Results show that IR-based quantitation provides results that match concentrations, determined by amino acid analysis, even in the presence of detergent or reducing agent. Also it is shown that, unlike colorimetric assays, the calculated protein concentration obtained from IR-based analysis is
unchanged regardless of the time delay between assay and data acquisition.
EMD Millipore Products Enable Customer Success
Many EMD Millipore products are used by Brian McFarlin at the University of North Texas. Dr. McFarlin has maintained his relationship with EMD Millipore because of our innovative products, outstanding customer service, and because the technologies developed by EMD Millipore in just the last 5 years have accelerated his research as shown in this video.
