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Clean-Up® Anion Exchange Columns

This sorbent is composed of a silica backbone bonded with a carbon chain terminated by a negatively or positively charged functional group. Ion exchange interactions occur between a sorbent that carries a charge and a compound of opposite charge. This electrostatic interaction is reversible by neutralizing the sorbent and /or analyte. Ion exchange bonds can also be disrupted by introduction of a “counter ion” to compete with…

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Description

This sorbent is composed of a silica backbone bonded with a carbon chain terminated by a negatively or positively charged functional group.

Ion exchange interactions occur between a sorbent that carries a charge and a compound of opposite charge. This electrostatic interaction is reversible by neutralizing the sorbent and /or analyte. Ion exchange bonds can also be disrupted by introduction of a “counter ion” to compete with the analyte for binding sites on the sorbent.

UCT offers a variety of both weak and strong anion exchange columns. Anion sorbents include aminopropyl, PSA, diethylamino, polyimine, and a selection of quaternary amines with various counter ions.

Application NoteSeparations

Determination of Anabolic Steroids in Horse Urine by SPE and LC-MS/MS

Anabolic steroids are used illegally in horse racing to enhance performance. The compounds are excreted in urine either as glucuronide or sulfated conjugate. To test for steroids in horse urine samples undergo an enzymatic deconjugation to release the parent compounds before extraction. Working with horse urine matrix is challenging and generally unpleasant.

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