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AXIMA Confidence Linear/Reflectron MALDI-TOF Mass Spectrometer

Shimadzu CorporationAvailable: Worldwide

High Sensitivity & Performance.

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Fast and reliable method for microorganism identification


Average Rating 5.0

Application Area:

Microbial identification

The AXIMA Confidence Linear/Reflectron MALDI-TOF Mass Spectrometer is able to be used for conventional proteomics and analysis of bio molecules, but another application is through the use of microbial identification. With high sensitivity, performance and robustness, it is excellent for routine analyses. The software is user-friendly, with a common user being able to perform experiments within a few weeks of training. For microbial identification, MALDI-TOF MS has proven to be a significant alternative to traditional methods of identification. This stems from the ease of use, minimal sample preparation, and it's time- and cost-efficiency. These results and identifications are reproducible and comparable to the traditional methods. This specific model has worked well in a lab of a few student users for groundwater analyses.

Review Date: 21 Mar 2017 | Shimadzu Corporation

An affordable, robust option for routine sample analysis performed manually or automatically, the AXIMA Confidence offers reliable mass information and MS/MS-derived structural detail. A linear mode enables outstanding levels of analysis of high-molecular weight samples while the reflectron mode, incorporating the patented curved-field reflectron, provides high resolution and mass accuracy.

Like all AXIMA MALDI-TOF systems, the AXIMA Confidence can be fully integrated with robotic sample preparation devices, including the AccuSpot MALDI plate spotter and CHIP chemical printer, to create application-specific platforms. Integration with the CHIP creates a powerful platform for tissue imaging, while using an AXIMA instrument with a Prominence GPC system and AccuSpot results in a fully automated platform for polymer analysis that delivers quick, detailed analysis of ultra-trace substances.


  • Near-axis laser irradiation with a 50Hz variable repetition rate N2 laser
  • Patented improved curved field reflectron
  • Mass range 1-500kDa in linear mode; 1-80kDa in reflectron mode
  • Mass resolution 5000 FWHM (ACTH 18-39) in linear mode; 15000 FWHM (ACTH 7-38) in reflectron mode
  • Sensitivity 500 amol (Glu-fibrinopeptide) in reflectron mode
  • Mass accuracy <10ppm in reflectron mode with internal calibration
  • Precursor ion resolution >200 FWHM
  • Seamless MS/MS utilizing PSD
  • Flexible target formats - ranging from 48 well microscope slide format targets to 384 well microtitre plate targets


  • Proteomics – Easy-to-use, intuitive Intellimarque™ software is designed with the flexibility to adapt to user workflows: from a handful of samples to high-throughput, fully automated data generation, data-dependent peptide mass fingerprinting and MS/MS for protein identification
  • Synthetic Polymers – Unique PolymerAnalysis™ software provides useful structural information and statistics in a text report format
  • Oligonucleotides – OligoAnalysis™ offers fully automated QC analysis of large numbers of oligonucleotides or peptides
  • Biomarker Discovery–Tissue Imaging – Protein/peptide biomarkers, drugs and their metabolites can be rapidly screened directly from tissue sections and their location mapped and visualized using integrated software tools

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