Products & ReviewLife Sciences

AlphaLISA

Alpha is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to target, a cascade of chemical reactions is set in motion, creating an amplified signal. These versatile beads are conjugated with various biomolecules that enable detection of unique biological events, offering maximum flexibility.

Revvity

The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our  Product Directory.

Average Rating 4.3

|1Scientists have reviewed this product

Ease of Use
After Sales Service
Value for Money
Write your own review

Average Rating 4.3

Application Area:

IL6 and IL10 quantification

We used the IL6 and IL10 AlphaLISA kits for a medium throughput screen and found the assays to be fast, easy and highly reproducible.

Review Date: 16 Jan 2015 | Revvity

Alpha technology is compatible with a wide range of sample types ranging from simple buffers, cell supernatants, and cell lysates to complex tissue, serum, plasma, and other biological samples.

Alpha offers benefits including:

  • Faster time to results
  • Ability to quantitate large protein complexes, nucleosomes, and viral particles
  • Option for multiplexing

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA SureFire®Ultra – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

Product Overview

Links