MP Biomedicals develops two new high-performance DNA extraction kits to advance both environmental and gut microbiome research
26 Aug 2020
MP Biomedicals have announced the launch of two new high-performance DNA extraction kits – SPINeasy™ DNA Kit for Soil and MagBeads FastDNA™ Kit for Feces – to advance microbiome research.
SPINeasy™ DNA Kit for Soil employs spin column technology, proprietary lysis buffers to protect the DNA and specially formulated buffers to remove inhibitors, to extract genomic DNA of high yield and purity from the diverse and difficult soil samples.
MagBeads FastDNA™ Kit for Feces allows high yield and quick isolation of genomic DNA from fecal samples. It is using proprietary magnetic beads with high binding capacity and selectivity for genomic DNA and can be applied on automated nucleic acid extraction instruments for high throughput processing.
Microbiome research has wide applications in agriculture, biofuel, ecology, environment remediation and healthcare. The characterization of soil and gut microbiome requires the extraction of genetic materials, but it is not an easy process as both sources have complex content which can hinder scientists from getting sufficient yield of DNA or DNA of high purity that can be used directly for downstream application.
“In the microbiome research, we understand that lack of consistency in DNA extraction process will hinder the accurate analysis of the microbial representation and the ability to compare results between studies.” explained Dr Wong Kak Ming, Research Scientist at MP Biomedicals.
Variability in results can stem from all the steps in the microbiome study process including sample preparation, DNA extraction, and downstream analyses. The development team at MP Biomedicals is focused on optimizing both the sample preparation and DNA extraction steps to ensure high quality and yield of DNA to advance the microbiome research.
Highlights on SPINeasy™ DNA Kit for Soil:
- Thorough lysis of cell bodies using Lysing Matrix E tube
- Lysis buffers to protect released DNA and remove unwanted RNA.
- Specially formulated buffers to get rid of humic substances and other inhibitors.
- Dedicated spin column S1 with high binding capacity and selectivity for genomic DNA.
- Consistently better yield and purity of DNA from various soil types as compared to other similar DNA extraction kits.
Highlights on MagBeads FastDNA™ Kit for Feces:
- Thorough lysis of cell bodies using Lysing Matrix E tube.
- Prewash buffers to effectively remove contaminants and inhibitors before lysis process.
- Lysis buffers to protect released DNA and remove unwanted RNA.
- Proprietary magnetic beads with high binding capacity and selectivity of the genomic DNA.
- Outstanding yield and purity of DNA from various fecal sample as compared to other similar DNA extraction kits.
- Works fast with manual operation using a magnetic rack and is also compatible with any open automation instruments when high throughput processing is needed.
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FastDNA SPIN Kit for Soil™
MP BiomedicalsIsolate Pure DNA from Cells Present in Soil or Other Environmental Samples! Thorough lysis in seconds of any microorganism present in environmental samples. Ready-to-use DNA for quantitative and qualitative characterization of microbial soil communities. Excellent reproducibility for an optimum assay-to-assay consistency. Total removal of humic acids and PCR inhibitors for a successful investigation of microbial diversity. The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungal, plant and animal genomic DNA from soil and environmental samples. Up to 500 mg soil are processed by the FastPrep®-24 5G or and any bead beating homogenizers with the Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive bacteria and yeast. The released DNA is purified by a silica-based spin filter method and is suitable for PCR and other downstream applications.
FastDNA™ Spin Kit for Feces
MP BiomedicalsThe FastDNA SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.
FastDNA SPIN Kit™
MP BiomedicalsRapid Method of Isolating Pure Genomic DNA from a Wide Variety of Sources! Isolate PCR-ready DNA from a variety of sample types No hazardous organic reagents are required The FastDNA™ SPIN Kit quickly and efficiently isolates genomic DNA from almost any sample (plant and animal tissues, cultured cells, bacteria, yeast, fungi, insects, etc). Up to 200 mg of tissue or cells are processed by the FastPrep-24™ 5G, FastPrep FP120™, FastPrep-96™ or SuperFastPrep-1™ with the Lysing Matrix A tubes. The kit includes 3 different chaotropic buffers for the homogenization of a wide variety of sample types and the released DNA is purified by a silica-based spin filter method. Purified DNA is ready for enzyme digestion, electrophoresis, PCR and any other desired application.