New RNA in situ hybridization application guide for single-cell analysis

Check out the new eBook guide that covers a wide range of higher-plexing applications, from neuroscience to immuno-oncology to cell therapy

10 May 2020
Edward Carter
Publishing / Media

Editorial article

RNA plays an important role in the regulation of cellular processes, making it an ideal indicator of dynamic gene expression changes in both healthy cells and disease phenotypes. Therefore, much like DNA and proteins, RNA is a powerful biomarker for understanding basic cellular functions, identifying optimal disease treatments, and developing new research tools.

As the need for increasingly accurate and biologically relevant data increases, this new eBook showcases the unique advantages of RNA in situ hybridization (ISH) technology.

The downloadable guide, outlining RNA-ISH assays with various levels of plexing, will present:

  • A series of case studies illustrating the research capabilities of high-plexing assays
  • Top tips on how to achieve accurate, reliable results for virtually any target species, tissue, or gene

Incorporating top tips from the experts, it also looks at how state-of-the-art RNA-ISH assays, such as RNAscope™ HiPlex Assays from Advanced Cell Diagnostics, can help researchers visualize gene expression within intact tissue samples at the single-cell level.

This excellent resource includes all the need-to-know information on higher-plexing your assays and can be downloaded free of charge here>>

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNARNA is a nucleic acid that plays a key role in gene expression and protein synthesis. It serves as a messenger between DNA and ribosomes, carrying genetic instructions to produce proteins. Advances in RNA-based therapies, such as mRNA vaccines and gene editing, have revolutionized treatment strategies for genetic disorders and infectious diseases. Explore RNA research tools and therapies in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.Single Cell AnalysisSingle-cell analysis involves studying individual cells to gain insights into their behavior, gene expression, and function. This approach is valuable in cancer research, stem cell biology, and immunology. Explore single-cell analysis products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.In Situ HybridizationMultiplexingMultiplexing refers to the ability to measure multiple targets or analytes simultaneously in a single experiment. This technique is valuable for high-throughput screening, diagnostics, and complex assays, as it increases efficiency and data quality. Browse our peer-reviewed product directory to find the best multiplexing products, compare tools, check reviews, and get pricing directly from manufacturers.