Waters Introduces Two New Column Chemistries for Protein Separations

27 Aug 2008

Waters Corporation announced today the introduction of new 300Å, reversed-phase columns for protein separations on traditional high performance liquid chromatographs or Waters ACQUITY UltraPerformance LC® (UPLC®) systems. The XBridge™ BEH300 C4, 3.5 µm and ACQUITY UPLC BEH300 C4, 1.7 µm columns deliver substantially greater protein resolving power and performance as compared to traditional 100-percent silica-based particle materials. These columns are now available for immediate delivery on a worldwide basis.

The first in a series of Protein Separation Technology columns, these columns are based on its second-generation, patented, highly-stable Ethyl Bridged Hybrid (BEH) particles that have set new standards for LC and UPLC separations. The results of accelerated stability tests confirm that the BEH300 C4 columns last longer and are more stable under low and high pH conditions than many competitive column offerings when tested under identical conditions. Other features of the BEH300 C4 columns include:

  • Separates proteins of various sizes, hydrophobicities, and isoelectric points yielding good peak shape
  • Surface chemistry that minimizes undesirable secondary interactions
  • Enhanced pH- and temperature-tolerant characteristics
  • Rigorously quality-control-tested with proteins to help ensure consistent batch-to-batch performance
  • Maintains protein resolution with LC/MS-compatible eluents

For additional information on ACQUITY UPLC BEH300 C4, 1.7 µm and XBridge BEH300 C4, 3.5 µm Columns, visit the company article page.

XBridge Columns

Waters

XBridge Columns include a selection of 10 general-purpose and application-specific sorbents that cover a wide range of particles sizes for analytical and preparative HPLC applications.   XBridge Column users can quickly develop robust methods using mobile phases throughout the entire pH range of 1-12. XBridge OBD™ Preparative Column users, benefit from high pH and temperature stability to provide increased mass loading for basic compounds.   Robust HPLC methods Seamless HPLC to UPLC® method transfer Preparative scaling for product isolation High pH stability Long lifetime and high reliability Maximum method development flexibility High chemical resistance

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ACQUITY UPLC BEH Columns

Waters

In 2004, Waters revolutionized liquid chromatography with the introduction of the ACQUITY UPLC System. At the heart of the system was Waters second generation hybrid, the Ethylene Bridged Hybrid (BEH) particle. In 2005, BEH Technology preparative particle sizes (2.5, 3.5, 5 and 10 μm) with the introduction of XBridge between HPLC and UPLC Technology platforms. BEH Technology particles offer: Excellent peak shape and efficiency for basic analytes A rational array of chromatographic selectivity Improvements in chemical stability at mobile phase extremes, particularly at elevated pH

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Waters Introduces Two New Column Chemistries for Protein Separations