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Thermo Fisher Scientific Introduces Alternative to Phospho-Antibodies for the Study of Phosphotyrosine Signaling

22 Apr 2009
Veronica Austin
Marketing / Sales

Product news

Thermo Fisher Scientific, the world leader in serving science, now offers the Thermo Scientific SH2 Domain Phosphotyrosine Capture Kits, fast and efficient tools for capturing specific tyrosine phosphorylated proteins from mammalian cell lysates. These new kits enable researchers to monitor and profile specific tyrosine phosphorylation events and identify specific SH2 domain protein-protein interaction networks.

The non-antibody-based assay uses GST-tagged SH2 domain peptides and glutathione agarose resin to capture and pull down the specific binding partners, providing an alternative to traditional immunoprecipitations using phospho-antibodies. The SH2 Domain Phosphotyrosine Capture Kits also offer an alternative to phosphoprotein enrichment resins that do not discriminate among proteins that are phosphorylated on serines, threonines or tyrosines. Subsequent analysis of the SH2 domain interactions can be performed using Western blots or mass spectrometry.

Kits for studying 17 specific SH2 Domains are available. Each kit contains 600 µg of a specific GST-SH2 domain and sufficient reagents (including negative controls) for six pull-down reactions with approximately 500 µg of lysate. The kits have a shelf life of one year.

For more information on the SH2 Domain Phosphotyrosine Capture Kits.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Thermo Fisher Scientific Introduces Alternative to Phospho-Antibodies for the Study of Phosphotyrosine Signaling