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Sensitive cfDNA sequencing using adaptive PCR control with icon96

n6 Tec., Inc
28 Apr 2026

This application note describes how adaptive, real‑time PCR amplification using the icon96™ thermocycler with AutoNorm™ technology enables highly sensitive sequencing of cell‑free DNA (cfDNA) from ultra‑low input samples. Developed in collaboration with the Dana‑Farber Cancer Institute, the workflow demonstrates uniform library yields across variable inputs, improved on‑target capture efficiency, and reliable detection of variants down to 0.5% allele frequency, even from just 1 ng of cfDNA.

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icon96™

n6 Tec., Inc

Stop gambling on cycle numbers. The icon96 uses iconPCR™ technology with AutoNorm™ software to provide real-time fluorescence feedback in each of 96 independent wells, preventing under-amplification dropouts and over-amplification artifacts—delivering balanced, sequencing-ready libraries without manual quantification or normalization.

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DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.cfDNATargeted Sequencing

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