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Recombinase Polymerase Amplification - The Breakthrough Isothermal Alternative to PCR

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TwistDx’s proprietary technology, Recombinase Polymerase Amplification (RPA), is transforming the ability to amplify and detect nucleic acids, in laboratory and resource-limited field settings

6 May 2017
Sonia Nicholas
Managing Editor and Clinical Lead

Editorial article

TwistDx's proprietary technology offers a rapid, efficient alternative to PCR. Watch the video to find out more. 

TwistDx’s proprietary technology employs recombinase enzymes which are able to pair oligonucleotide primers with homologous sequence in duplex DNA. DNA is directed to defined points in a sample DNA, and if the target sequence is present, DNA amplification is initiated, with no need for thermal or chemical melting techniques. The DNA reaction is rapid and results in specific DNA amplification to a detectable level in approximately 3-10 minutes.

RPA is more rapid, convenient and efficient than the traditional polymerase chain reaction (PCR). Not only that, but the method is portable, with no need for a thermocycler, trained technician or a laboratory environment.

RPA is currently being used for a wide variety of applications including infectious diseases, plant biology, point-of-care and biodefense. You can find a list of over 150 peer-reviewed publications on the TwistDx website.

Infectious disease diagnostics

Watch this video to hear how scientists at the Georg-August-University in Germany are using RPA to develop portable RPA-based tests for Ebola, Zika and Chikungunya. The assays are as sensitive and specific as laboratory PCR-based detection assays, and they provide results in just a few minutes.

Fast Diagnosis of Ebola, Zika and Chikungunya Using Recombinase Polymerase Amplification

Biodefence applications

Watch this video to hear how scientists from the Universitat Rovira i Virgili’s Interfibio Research Group in Spain, are using RPA to detect potential biowarfare agents. This research is proof of concept for applying RPA in a heterogenous format, with one primer immobilized onto a solid surface.

Detecting Bioterrorism Threats Using an Enzyme-Linked Oligonucleotide Assay Approach

The TwistAmp® Basic contains all enzymes and reagents necessary for the amplification of DNA, so that the user only needs to supply the primers and the template. The new improved formulation provides rapid DNA amplification, single-molecule detection and high sensitivity.

Find out what TwistDx customers think of the TwistAmp technology:

View product details for the TwistAmp® Basic

View the TwistDx product portfolio

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Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Point-of-CarePoint-of-Care Testing (POCT) or Near Patient Testing (NPT) products are available for urine, blood and other clinical chemistry analyses. POCT includes: blood glucose testing, blood gas and electrolytes analysis, rapid coagulation testing (PT / INR), rapid cardiac markers diagnostics, drugs of abuse screening, urine strips testing, pregnancy testing, fecal occult blood analysis, food pathogens screening, hemoglobin diagnostics, infectious disease testing and cholesterol screening.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. 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Recombinase Polymerase Amplification - The Breakthrough Isothermal Alternative to PCR