Quality control in the PacBio HiFi whole genome sequencing library preparation workflow
3 Nov 2025Accurate and reliable next-generation sequencing (NGS) results rely on starting with high-quality samples. In long-read sequencing workflows, such as the PacBio HiFi workflow, essential quality control (QC) steps include assessing input gDNA, intermediate steps throughout library preparation, and the final library. Electrophoretic analysis enables evaluation of sample size, purity, and integrity at each of these steps. Agilent offers several automated electrophoresis systems, including the Agilent Femto Pulse, Fragment Analyzer, and TapeStation systems, each with unique benefits for DNA QC.
This application note demonstrates the capabilities and limitations of each of the automated electrophoresis systems within the PacBio HiFi whole genome sequencing (WGS) library preparation workflow, aiding in the selection of the appropriate QC system.
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Femto Pulse System
Agilent TechnologiesThe Agilent Femto Pulse system delivers remarkable sizing and sensitivity to researchers.
Fragment Analyzer Systems
Agilent TechnologiesThe Agilent Fragment Analyzer systems are fluorescence-based capillary electrophoresis instruments for the accurate sizing, quantification, and qualification of nucleic acids (DNA and RNA).
4150 TapeStation system
Agilent TechnologiesThe 4150 TapeStation system is an economic, entry-level system which comes with data processing software, reagents, and ScreenTape devices for analysis of sample size, quantity, and integrity of 1 to 16 samples. Delivering highly accurate and precise analytical evaluation, the systems fit perfectly into next-generation sequencing (NGS) or biobank workflows for handling smaller numbers of samples.