Unlocking superior microbial profiling with iconPCR

27 Feb 2026

16S rRNA gene sequencing is essential for exploring microbial diversity in various ecosystems, including soil, water, and human associated microbiomes. However, conventional PCR methods used in 16S library preparation often introduce significant biases due to overamplification, under-amplification, and the generation of chimeric sequences.

Discover how the icon96™, developed by n6, represents a transformative shift in PCR technology. Summarized in this comparative study, learn how using icon96 with iconPCR and conventional PCR workflows can produce high data quality, taxonomic resolution, and workflow consistency, especially when paired with long-read platforms like PacBio for full-length amplicon sequencing.

icon96™

n6 Tec., Inc

Stop gambling on cycle numbers. The icon96 uses iconPCR™ technology with AutoNorm™ software to provide real-time fluorescence feedback in each of 96 independent wells, preventing under-amplification dropouts and over-amplification artifacts—delivering balanced, sequencing-ready libraries without manual quantification or normalization.

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