Optimize your single cell experiments with iconPCR

27 Feb 2026

Standard PCR systems require users to set a predetermined number of cycles, based on assay and sample-specific factors. For single cell sequencing experiments, this requires knowledge of how many cells are captured and the RNA content within the cells. Different cell numbers and RNA quality require careful curation of the PCR cycle numbers for each sample, necessitating multiple thermocycler runs, which increases workflow complexity and potential sources of error.

Discover how the icon96™, when used together with AutoNorm™ can monitor each individual PCR reaction and terminate in real time based on fluorescence thresholds. This eliminates the guesswork of traditional workflows and ensures every library is amplified optimally.

icon96™

n6 Tec., Inc

Stop gambling on cycle numbers. The icon96 uses iconPCR™ technology with AutoNorm™ software to provide real-time fluorescence feedback in each of 96 independent wells, preventing under-amplification dropouts and over-amplification artifacts—delivering balanced, sequencing-ready libraries without manual quantification or normalization.

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