ResourceLife Sciences
Optimizing DNA fragmentation and NGS library preparation with automated liquid handling
30 Jun 2026Next-generation sequencing (NGS) library preparation depends on precise, repetitive pipetting, yet manual fragmentation, polishing, ligation and clean‑ups can introduce variability and slow throughput. These steps demand consistency to ensure high‑quality libraries, but hands‑on workflows often limit efficiency and reproducibility.
Explore this resource to see how liquid handling for the sparQ DNA Frag & Library Prep Kit is automated using the ASSIST PLUS pipetting robot. Validated, ready‑to‑use VIALAB programs streamline enzymatic fragmentation, adapter ligation and magnetic‑bead clean‑ups, enabling fast, reproducible library preparation with minimal manual intervention.
Resource details:
Resource type: Application note
Page count: 10
Read time: 5 mins
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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library PreparationLibrary preparation is a critical step in sequencing workflows, where DNA or RNA samples are converted into libraries for high-throughput, next generation sequencing. This step ensures accurate results and minimizes biases. Explore library preparation kits in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.