New and Improved ChIP Assay Kit Released
1 May 2017
Chromatrap® has introduced a new and improved version of it’s popular solid phase chromatin immunoprecipitation (ChIP) assay kit for chromatin extracted from Formalin Fixed Paraffin Embedded (FFPE) tissue.
Ideal for tissue samples, biopsies and difficult to lyse samples, the Chromatrap® FFPE ChIP-seq kit has overcome the issues often associated with FFPE ChIP, by offering a streamlined protocol which provides the user with excellent quality isolated chromatin.
The new updated Chromatrap® FFPE ChIP-seq kit protocol is shorter, offers increased chromatin yield / accessible epitopes and provides greater flexibility in chromatin sample loading for ChIP.
The sensitivity of the unique Chromatrap® solid state platform ensures that small samples can be used with efficient enrichment of both high and low abundant targets. Chromatrap® FFPE ChIP-seq kits provide a quicker, easier and more efficient way of performing ChIP assays from a range of FFPE samples. Using the unique, patented Chromatrap® technology maximises the capture efficiency of the target chromatin/antibody complex. By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss.
This kit is compatible with both qPCR and sequencing as a downstream process and has a wide dynamic range, ensuring that adequate DNA is enriched for library preparation for sequencing. The wide dynamic range of the Chromatrap® FFPE ChIP-seq kit enables better results from small sample sizes and provides greater flexibility. Prior to sequencing, DNA purification is required and Chromatrap® purification columns are supplied for ease of use.
Each Chromatrap® FFPE ChIP-seq kit contains sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.
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Chromatrap® FFPE ChIP Kit
ChromatrapEpigenetic identification and targeting in clinical FFPE tissues is a crucial step for potential epigenetic therapeutics and personalized medicine therapies. FFPE is a standard method to archive and preserve medical tissue biopsy samples. These archives, kept in many hospitals world-wide, are a huge, rich and untapped sample resource for genetic research. Due to the cross-linking of proteins by formaldehyde, FFPE tissues present a particular challenge for ChIP analysis. In addition, the small size and delicate nature of the tissue, and the difficulty in extracting samples from paraffin, used to provide tissue support after preservation, can damage complex cellular complexes such as chromatin. ChIP assays from these samples have therefore proved time consuming and difficult. Chromatrap® FFPE ChIP kit overcomes these issues and provides the following benefits: Provides greater flexibility and more IPs per sample Works across a range of FFPE samples, human and animal Available in 96-well plate or spin column format Works equally well with high and low abundant marks Provides sufficient DNA to perform library preps for ChIP-seq assays