Millipore Launches Fast-Trap™ Virus Purification Kits
8 Apr 2008Millipore Corporation, a Life Science leader providing technologies, tools and services for bioscience research and biopharmaceutical manufacturing, today announced the availability of its two Fast-Trap Virus Purification and Concentration Kits, one for adenovirus purification and one for lentivirus purification. Purifying virus after propagation in host cells is a critical step towards using the viruses as vectors for genetic modification of target cells. The Fast-Trap Virus Purification and Concentration Kits contain all the components to accommodate the entire virus purification workflow.
The Fast-Trap kits provide results that are more reliable in substantially less time than traditional methods by incorporating Millipore’s Steriflip® filter unit, an easy-to-use format utilizing a new anion exchange membrane that binds and elutes virus. The vacuum-based, closed-system purification kits are quick, easy, and safe to operate with a turnaround time of less than two hours compared to two days with traditional purification techniques.
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Fast Trap Adenovirus Purification and Concentration Kit
MerckThe Fast-Trap Adenovirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification of ≥ 3 mL of adenovirus serotype 5, up to a capacity of approximately 1x1013 total adenovirus particles. The purification kit protocol calls for releasing the virus from the infected cells using multiple freeze-thaw cycles, then collecting the crude adenovirus. Most of the large cellular debris is removed by centrifugation, leaving the viable virus particles in the supernatant. The supernatant is further clarified by passing it through a Steriflip®-HV 0.45 µm filter. After adding a binding buffer, the virus solution is passed over a treated filter that adsorbs the virus particles, allowing the smaller cellular debris and media to pass through the filter. Following a wash to remove any weakly bound proteins, the virus is eluted off the filter with an elution buffer. Finally, the virus can be concentrated and exchanged into the desired buffer using an Amicon® Ultra centrifugal filter unit. For laboratory use only; not for diagnostic or in vivo use.
Fast Trap Lentivirus Purification and Concentration Kit
MerckThe Fast-Trap Lentivirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification of ≥ 3 mL of lentivirus, up to capacity of approximately 2x108 total infectious lentivirus particles. The purification kit protocol calls for collecting the supernatant from transfected cells that have released the virus. Most of the large cellular debris is removed by centrifugation, leaving the viable virus particles in the supernatant. The supernatant is further clarified by passing it through a Steriflip®-HV 0.45 µm filter. After adding a binding buffer, the virus solution is passed over a treated filter that adsorbs the virus particles, allowing the smaller cellular debris and media to pass through the filter. Following a wash to remove any weakly bound proteins, the virus is eluted off the filter with an elution buffer. Finally, the virus can be concentrated and exchanged into the desired buffer using an Amicon® Ultra centrifugal filter unit. For laboratory use only; not for diagnostic or in vivo use.