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Millipore Announces Protocol for the Concentration of Genomic DNA for Forensic Analysis

Merck
1 Dec 2006

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Millipore Corporation today announced the availability of a faster protocol using Amicon® Ultra-4 100K NMWL centrifugal filter devices to concentrate human genomic DNA to be used in forensic analysis.

Amicon Ultra-4 devices can quickly ultrafilter dilute nucleic acid samples for fast separation of genomic DNA from low molecular weight compounds and analysis inhibitors.

The source material for forensic analysis is typically blood or blood stains, bodily fluids and/or tissue samples obtained either from crime scenes, directly from individuals, or from human remains. The isolated genomic DNA is used to identify individuals based on their Short Tandem Repeat (STR) profile. The resulting STR genetic fingerprints are unique to each person and can provide unequivocal identification of an individual.

The Amicon Ultra device design is compatible with the multiple spin recovery assays used for STR analysis. The concentrated DNA is collected from the filter unit sample reservoir, while the ultrafiltrate can be collected in the provided centrifuge tube if desired. The Amicon Ultra centrifugal devices incorporate the Ultracel® low-binding ultrafiltration membrane in a vertical housing design to deliver high sample recoveries with 10-minute spin times. In addition, Amicon Ultra devices allow for direct pipette sample access and are compatible with most rotor types. Amicon Ultra-4 devices are available in packs of 8, 24, and 96 filter units, which come assembled with centrifuge tubes and caps.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.FiltrationFiltration and microfiltration are used to separate solids, particulates or large molecules from fluids (liquid or gases) in order to purify the filtrate. Explore a range of filter formats, including filter paper, plates and membranes for your separation needs. While larger particulates may be separated by gravity filtration, smaller particulates may require additional force for timely separation. Microfiltration equipment includes vacuum filters, as well as positive pressure, crossflow and centrifugal filtration devices. Find the best filtration products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.CentrifugesCentrifuges are used to separate particulates suspended in a liquid via the application of a centrifugal force including cells, macromolecules, nanoparticles and precipitates. Centrifuges come in three sizes: microcentrifuges, benchtop and floor-standing centrifuges, depending on the volume and number of samples that require separation. When choosing a centrifuge, consider its maximum separation power indicated by its relative centrifugal force (RCF) value. Low force centrifuges reach a maximum RCF of 8,000 g, high-force centrifuges can reach maximum RCF values of 80,000 g. For extremely high forces, consider ultracentrifuges (up to 800,000 g). Refrigerated and vacuum centrifuges also known as concentrators are available for more specialized applications. A range of volumes are available for centrifuge tubes and microfuge tubes, depending on sample sizes. Find the best centrifuges in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Millipore Announces Protocol for the Concentration of Genomic DNA for Forensic Analysis