Manual Pipetting of 384-Well Plates Made Easy with Eppendorf

The new multichannel pipettes can now tackle entire columns and rows of a 384-well plate in one single step

6 May 2019
Charlie Carter
Life Sciences Editor

Once throughput increases and sample volumes decrease, users face a dilemma. The so-called “alternate well pipetting method”, which uses 8 and 12 channel pipettes to fill 384-well plates, demands intense concentration; furthermore, it is time-consuming and carries a high risk of error.

With their 16 and 24 channels, the new mechanical multichannel pipettes Eppendorf Research plus and the new electronic multichannel pipettes Eppendorf Xplorer plus can now tackle entire columns and rows of a 384-well plate in one single step. Up to 24 reactions may thus be started and stopped simultaneously. An entire plate can be managed manually within the space of a minute. In this way, the user will save time while at the same time improving the reproducibility of their results.

The system that comprises the innovative Eppendorf pipette tips epT.I.P.S.® 384 and ep Dualfilter T.I.P.S.® 384 promise the user the utmost security. The unique SOFTattach technology utilizes elastic forming grooves that contribute to a perfect tip fit as well as to a perfect seal of the system. The tip attachment forces could thus be reduced by an additional 40% per cone. An extremely fine tip shape that displays perfect coaxiality makes secure manoeuvring of samples into the tiny wells of a 384-well plate as easy as pie. With these innovations, the Eppendorf PhysioCare Concept® has once again achieved a significant leap forward, and even under conditions of increased throughput, the pipetting experience has now become even more ergonomic and comfortable.

Do you use any Eppendorf products in your lab? Write a review today for your chance to win a $400 Amazon gift card >>

Pipette tips - ep Dualfilter T.I.P.S.

Eppendorf

There are certain situations in the lab in which suitable safety precautions must be taken in counteract any potential risks and to prevent any unpleasant surprises. It must be possible to anticipate in advance the dangers presented by carryover effects from radioactive, highly toxic or infectious sample material as well as the contamination risk caused by foreign DNA (e.g. as a result of aerosol formation).Our ep Dualfilter T.I.P.S. have an integrated dual filter made of pure hydrophobic polyethylene without "self-sealing" additives. This filter provides protection against carryover and contamination caused by aerosols and does not swell up as a result of incorrect pipetting. Samples can then be easily recovered. In line with the special biological purity requirements of a PCR application, ep Dualfilter T.I.P.S. are "PCR clean", sterile (in accordance with USP, DAB and Ph.Eur.) and thus free of human DNA, DNase, RNase and PCR inhibitors. The new Eppendorf ep Dualfilter T.I.P.S. are the first filtertips with a two phase filter for contamination protection. The tips are manufactured to the highest possible quality from pure, non recycled materials under cleanroom conditions. The are characterized by defined flow dynamics, low wettability and high thermal stability. They are certified pyrogen free and PCR clean (free from human DNA, DNase, RNase and PCR-inhibiting substances). The two-phases effect! Two filter layers provide dual protection for pipette and sample Two filter layers provide dual protection from aerosols and biomolocules Free from PCR inhibitor additives Patent pending technology All tips are provided sterile, Eppendorf PCR clean and pyrogen-free Batch certified ep Dualfilter T.I.P.S. contains well-defined different pore sizes within the two filter layers. The white layer which faces the sample retains drops, splashes and aerosols. the blue layer above it forms a highly-effective double barrier, that reliably binds even biomolecules. Even more, ep Dualfilter T.I.P.S. are characterized by: Defined flow dynamics Low wettability High thermal stability Leak proof seal* Minimum attachment and ejection forces* *when used in combination with Eppendorf pipettes

(5)

Pipette tips - epTIPS

Eppendorf

epTIPS from Eppendorf epTIPS - With the new generation of highly-accurate pipette tips, Eppendorf is taking the new standards of contemporary laboratory practice into account. All over the world, scientists and laboratory staff are using ever more accurate dispensing devices to try and satisfy the stringent requirements of reliability, reproducibility and the trend towards smaller sample volumes. A pipette is only as good as its tipOnly when using perfectly manufactured pipette tips can the precision of modern pipettes be fully exploited. With the new generation of Eppendorf pipette tips, you can make even better use of this potential. The new epTIPS - Totally Integrated Pipetting System - have been perfectly matched to Eppendorf pipettes. This results in consistently reduced tip attachment and ejection forces, while ensuring complete sealing. This concept guarantees that Eppendorf pipettes are consistently developed and manufactured to address both physiological and ergonomic concerns, from the button to the pipette tip. Additionally, the new Eppendorf epTIPS can still be used with pipettes from other manufacturers.State of the art production techniquesWe have looked at, optimized or completely redesigned every detail of our well-known tip system. The material and its properties, fit, design and the forces required for operation, with the strictest quality standards being given top priority. A modular system consisting of a variety of purity levels and different types of packaging, such as bagged products, boxes, refill trays and single wrapped tips, provides more flexibility than ever.An overall concept was developed to combine every conceivable application and volume with perfect functionality. epTIPS: as reliable as ever, and more progressive than ever before.

(17)

Links

Tags

Manual Pipetting of 384-Well Plates Made Easy with Eppendorf