Labcyte Awarded 28th U. S. Patent Describing Improved HTS Microplates for Low Volume Acoustic Transfer

16 Mar 2007

Product news

Labcyte Inc. announces the issuance of U.S. Patent 7,185, 969 describing microplates used in high-throughput screening that have low electrical resistance. These plates are more easily electrically grounded than traditional plastic microplates and are more easily de-ionized.

“The trajectory of small droplets can be influenced by electric charges and fields,” said Chief Technical Officer, Richard Ellson. “We deionize both source plates and destinations in our current instruments, the Portrait™ 630 reagent multi-spotter, the Echo® 550 and Echo 555 liquid handlers to reduce this effect. These advances in microplates will reduce the time required for deionization and improve the productivity of the process.”

“The Labcyte acoustic droplet ejection (ADE) technology used in our award-winning Echo® Series 500 liquid handlers has quickly become the state-of-the-art in sample transfer in high-throughput screening laboratories in the pharmaceutical industry. The technology described in this patent will allow us to increase throughput while expanding the range of available applications. This invention will also facilitate the placement of arrays onto the bottom of individual wells in multi-well systems. In this case, it is not sufficient to ensure that the compound was simply transferred to a well but that it was transferred to a particular location in the well with no overlap with other compounds transferred. Even a slight charge could force droplets to change trajectory and position of or even move after they land.

These microplates will aid in miniaturization and enable our users to take further advantage of the small drops produced by our acoustic drop ejection technology.

Labcyte Inc., headquartered in Sunnyvale, California, is the world leader in providing acoustic droplet ejection technology for pharmaceutical and life science applications. The award-winning Echo 500 series liquid handlers and Portrait 630 reagent multi-spotters are used in seven of the 10 largest pharmaceutical companies, as well as in leading academic and research institutions and contract research organizations worldwide. The Labcyte acoustic droplet ejection technology has broad applications including compound management, assays, arraying, particle manufacturing, imaging mass spectrometry, and live-cell transfer. Labcyte also provides a range of unique microplate consumables. Labcyte has 28 issued U.S. patents, 3 issued European patents and additional international filings.

Echo® 650 Acoustic Liquid Handler

Beckman Coulter Life Sciences

Enables assay miniaturization in a broad range of applications. Transfer from 384-well and 1536-well microplates. Accurate, precise, contact-free acoustic transfer in volumes as low as 2.5 nL Transfer from Echo Qualified sample tubes* and microplates.

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Echo® 555 Liquid Handler

Beckman Coulter Life Sciences

Echo® 555 liquid handler - the world's fastest acoustic dispensing platform for life science applications. The Echo® 555 liquid handler is the world’s fastest acoustic liquid handling platform for life science applications. Laboratories that demand very high throughput rely upon the Echo 555 liquid handler for round-the-clock trouble-free operation to enable the fastest discovery.The Echo 555 doubles the throughput of the Echo 550 and provides transfers from as many as 660,000 wells per day. Like the Echo 520 and Echo 550 liquid handlers, the Echo 555 uses "touchless" ADE transfer technology. ADE uses sound energy to transfer 2.5 nL droplets from Echo-qualified 384- and 1536-well source plates into 96-, 384-, 1536- and 3456-well destination plates.This unique touchless technology eliminates consumable tip costs, washing, and cross-contamination. Unsurpassed volumetric precision (≤ 8% CV) and accuracy (≤ 10% CV) ensure superior results. Independent studies have shown that Echo liquid handlers eliminate false negatives due to sample loss on tip surfaces in low-concentration transfers. Like the Echo 550 liquid handler, the Echo 555 liquid handler reports both fluid volume and DMSO hydration.Echo® 555 Liquid Handler Features: Tipless transfer, resulting in dramatic cost savings Accurate, precise movement of nanoliter-scale quantities, enabling assay miniaturization and further reducing costs Elimination of cross-contamination, carryover or leachates Better results through direct dilution of compounds, an improvement over serial dilution methods Improved information about sample integrity and improved transfer quality through measurement of DMSO hydration level Applications: Compound management Compound screening siRNA screening Biochemical assays Cell-based assays PCR Genotyping Gene expression

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Labcyte Awarded 28th U. S. Patent Describing Improved HTS Microplates for Low Volume Acoustic Transfer