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How to determine Pancreatic Elastase 1 with Eppendorf epMotion<sup>®</sup>

Eppendorf
12 Jun 2007

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A new application describes how Eppendorf epMotion automated pipetting system can be used for the determination of human pancreatic elastase 1. The ScheBo® Pancreatic Elastase 1 Stool Test can be performed semiautomated or fully automated with the help of epMotion 5070 or 5075. The ScheBo ELISA test kit is based on monoclonal antibodies, and it is applied in the diagnosis of pancreatic diseases.

With the epMotion, all washing, dispensing and aspirating steps are performed automatically. After the epMotion program is finished, the optical density is measured with a regular micro test plate reader, and the results are evaluated with standard ELISA software.

The automated performance of the ScheBo Pancreatic Elastase 1 test in combination with epMotion results in a reliable determination of pancreatic elastase 1 in stool. Due to the automation process, a significant amount of time can be saved when working with epMotion.

To download the Application Note "Determination of Pancreatic Elastase 1 on epMotion 5070 or 5075"(Note 138), please view the article webpage.

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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Lab-on-a-ChipLab-on-a-chip (LOC) technology uses microfluidic chips, such as microarrays, to perform biochemical assays on a small scale. Benefits of lab-on-a-chip include the small volume of sample required as well as the speed of analysis. In selecting lab-on-a-chip devices consider the assay, cost, reliability, sensitivity and signal to noise ratio.Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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How to determine Pancreatic Elastase 1 with Eppendorf epMotion<sup>®</sup>