High Performance ChIP-Seq kit for Fresh & Frozen Tissue Samples
2 Feb 2018
Porvair Sciences has announced a new and improved Chromatrap® ChIP-Seq kit (v.1.5) that is optimized to work from fresh and frozen extracted tissue as well as cell lines.
This latest version of the popular Chromatrap® ChIP-Seq kit enables ChIP to be performed from as little as 50mg of a wide range of extracted tissue samples. The new protocol incorporates optimized lysis and hypotonic buffers and guidelines on how to achieve maximum extraction of chromatin from limited sample quantities. This ground-breaking performance, that delivers great results whilst saving your precious samples, is further supported by a number of recent research publications.
Review the latest Chromatrap® ChIP-Seq kit protocols >>
For labs using FFPE tissue, Porvair Sciences also offers an optimized Chromatrap® FFPE kit
Chromatrap® ChIP-Seq kits utilize a proprietary solid-state technology to deliver a streamlined ChIP-Seq protocol from small cell numbers and low chromatin concentrations. Specifically adapted for broader chromatin concentrations, Chromatrap® ChIP-Seq combines the dynamic range of Chromatrap® with the downstream analysis power of deep sequencing. This allows faster, more reproducible genome wide identification of transcription factor binding sites and specific DNA associated protein modifications. The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors.
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Chromatrap® Native ChIP Kit
ChromatrapChromatrap® Native ChIP kits provide an alternative to traditional cross-linked ChIP, which removes the need for fixing cells prior to extraction.
Chromatrap® FFPE ChIP Kit
ChromatrapEpigenetic identification and targeting in clinical FFPE tissues is a crucial step for potential epigenetic therapeutics and personalized medicine therapies. FFPE is a standard method to archive and preserve medical tissue biopsy samples. These archives, kept in many hospitals world-wide, are a huge, rich and untapped sample resource for genetic research. Due to the cross-linking of proteins by formaldehyde, FFPE tissues present a particular challenge for ChIP analysis. In addition, the small size and delicate nature of the tissue, and the difficulty in extracting samples from paraffin, used to provide tissue support after preservation, can damage complex cellular complexes such as chromatin. ChIP assays from these samples have therefore proved time consuming and difficult. Chromatrap® FFPE ChIP kit overcomes these issues and provides the following benefits: Provides greater flexibility and more IPs per sample Works across a range of FFPE samples, human and animal Available in 96-well plate or spin column format Works equally well with high and low abundant marks Provides sufficient DNA to perform library preps for ChIP-seq assays