Corning<sup>®</sup> Introduces the HYPERFlask™ Cell Culture Vessel: A Revolutionary Multi-tier Flask
5 Jun 2007Corning Incorporated, a global supplier of scientific laboratory and drug discovery products for more than 90 years, introduces the HYPERFlask™ vessel for high density cell culture.
The HYPERFlask Cell Culture Vessel is a revolutionary new design that provides ten times more surface growth area than the standard T-175 cm2 flask, with the same external dimensions.
The HYPERFlask vessel is designed for manual use as well as for use in The Automation Partnership SelecT™ automated cell culture system. The flask consists of ten equivalent layers or ‘flaskettes’, each containing a gas permeable cell growth layer, that are joined together to form a multi-layered cell culture vessel. The HYPERFlask vessel incorporates an air gap between each flaskette for optimal gas exchange and each flaskette is treated with the patented Corning CellBIND® Surface for optimum cell attachment and growth.
Cell Culture Scale Up Using a Single HYPERFlask Vessel (100-200 x 106 cells/flask):
- Freeze 100 cryovials of cells from the same passage (1-2 x 106 cells/vial)
- Plate 100 – 384 well plates for a cell based assay (5000 cells/well)
- Transfect 100-200 x 106 cells in a single flask
- Examples of cells seeded in the HYPERFlask vessel
• CHO-K1
• CHO (transfected)
• HEK293
• MRC5
• A459
• A375
• CV-1
• PBMC
• IMR-32
• Normal human dermal fibroblasts
• Vero
• HT1080
The HYPERFlask cell culture vessel increases cell culture productivity and capacity by reducing processing time and saving on incubator space. Cells harvested for cell based assays have demonstrated similar pharmacology to cells grown in more traditional cell culture vessels. In addition, measuring pH, glutamate, glutamine, potassium, sodium, lactate, ammonia, glucose and osmolarity demonstrated similar cellular metabolism with cells harvested from both cell culture vessels.
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Corning® CellBIND® Surface HYPERFlask® Cell Culture Vessel
Corning Life SciencesThe High Yield PERformance Flask (HYPERFlask) cell culture vessel has 1720 cm2 available growth area on a polystyrene gas permeable surface. Treated for optimal cell attachment and gamma sterilized. Bar coded and compatible for use on Cell Culture Automation Platforms. Solid cap with liner on a straight neck designed for filling with growth medium. Made with USP Class VI Materials – molded and assembled in a Certified Class 100,000 clean room Sterile – gamma irradiated to sterility assurance level (SAL) 10⁻⁶ Clean Room Packaging – each pack is double bagged. Innovative 10Layer Design – 10 interconnected polystyrene growth surfaces Optimal Growth – Corning CellBIND® Surface treated gas permeable polystyrene for superior cell attachment and growth Increase Cell Yield – 10-fold higher cell yield increases productivity and capacity Time and Space Savings – Reduce processing time and incubator storage space by handling one flask compared to 10 traditional 175 cm2 flasks