Carl Zeiss Offers Free Colour Selection for LSM 710
16 Jul 2009The explosion in the number of new fluorescent dyes has opened up exciting new opportunities for life science researchers. However, each requires the microscope system to be equipped with an appropriate excitation laser, a limitation that has greatly restricted their adoption.
According to Carl Zeiss, the answer is the new In Tune laser system, which offers free selection of laser lines in the range 488 nm to 640 nm. Together with the LSM 710 laser scanning microscope, In Tune enables cell biologists, physiologists and researchers in biology, medicine and biophysics to perform novel fluorescence measurements for the first time. Whatever the excitation wavelength required, In Tune matches the dye perfectly to enable their use in intensity or lifetime imaging experiments.
The choice of fluorescent dyes is unrestricted as In Tune can be used alongside other system lasers, from near UV to far red. This is particularly important for FRET (Förster Resonance Energy Transfer) as In Tune allows the unrestricted use of new dye combinations in the green-red spectral range. With its 40 MHz pulse repetition rate In Tune is also an ideal source of excitation for FLIM (Fluorescence Lifetime Imaging Microscopy) experimentation.
In Tune is as stable and reliable as standard gas or diode lasers, offering a comparably low noise level that does not degrade the high image quality of the LSM 710. New or existing LSM 710 or LSM 710 NLO systems can be equipped with In Tune and control of the new laser is integrated into the Zeiss ZEN software where ‘Smart Setup’ takes care of the LSM settings for excitation and detection.
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ZEISS LSM 710 for Fluorescence Imaging
ZEISS Research Microscopy SolutionsExperience freedom with your dyes and applications The new illumination and detection design of LSM 710 brings complete freedom to your fluorescence microscopy. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. LSM 710 enables confocal microscopy for a wide variety of applications. With the inverse Axio Observer from Carl Zeiss, LSM 710 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard. LSM 710 gives you flexibility, allowing you to perform confocal microscopy in exactly the way you need. Features: Oversampling with 30 percent longer sampling time per pixel for low noise Excellent contrast characteristics, even with strongly reflective samples – thanks to laser suppression that has been improved by 100-1000x A huge range of QUASAR detector configuration options – to meet your requirements The innovative grid and spectral recycling loop design ensures that not even a single valuable photon will be lost from your sample. Up to 80 percent dark noise reduction – thanks to the QUASAR array detector by Carl Zeiss 34-channel parallel imaging – across the complete wavelength range APD imaging with photon counting Low noise electronics and lasers