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Biognosys’ Spectronaut™ Proteomics Software Now Supports Data-Independent Acquisition on Thermo Scientific™ Q Exactive™

28 Aug 2013
Sarah Thomas
Associate Editor

Product news

Biognosys AG, a Swiss Proteomics Company, has announced the second release of its Spectronaut™ software for analysis of Hyper Reaction Monitoring (HRM) data. HRM is a targeted proteomics technology that enables reproducible and accurate quantification of 1000s of proteins in a single instrument run. It thereby bridges the gap between the scale of shotgun proteomics with the ability to identify many proteins and the quantitation accuracy of MRM/SRM that allows high quality quantification of smaller number of proteins.

HRM is based on data-independent acquisition (DIA) performed on the new generation of mass spectrometric systems. DIA on the Thermo Scientific™ Q Exactive™ hybrid quadrupole-Orbitrap mass spectrometer enables acquiring MS/MS signals from all detected precursor ions present in the sample similar to SWATH™ Acquisition performed on AB SCIEX TripleTOF® 5600+ System. HRM combines DIA with high resolution MS/MS, large spectral libraries and a targeted approach for data deconvolution and analysis.

Spectronaut™ software was first launched by Biognosys in January 2013 for analysis of SWATH data. The new software release also supports analysis of DIA data generated on Q Exactive. Together with this release Biognosys provides an acquisition method for Q Exactive that has been optimized for high content quantification experiments.

Other new features of this Spectronaut™ release include advanced HRM-specific peak scoring and new visualization options like a multiple-run alignment view. Spectronaut™ uses a proprietary high speed data format that allows processing of 100’000s transitions within minutes. Furthermore, Spectronaut™ incorporates advanced mProphet algorithms (Nature Methods, 2011) adapted for HRM to enable accurate signal detection and statistically valid peak scoring. The powerful principle behind Spectronaut™ is the integration of the software algorithms with a reagent kit – HRM Calibration Kit – that provides optimal customized parameter calibration for any end-user system independently on the specific chromatography and mass spectrometer set-up. Spectronaut™ is a user-friendly desktop application that can be installed and run on a standard notebook.

Biognosys currently offers its HRM technology as a service for discovery applications and highly multiplexed protein quantification. With the second Spectronaut™ release that is free for academic researchers Biognosys expands its customer base to Q Exactive users facilitating further adoption of HRM technology and supporting open innovation and collaboration between applied and academic research.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

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