Bio-Rad Announces New Human Primary Cell Transfection Protocols

Bio-Rad Laboratories, Inc. a multinational manufacturer and distributor of life science research and clinical diagnostic products, announced today 12 new protocols for electroporation of human primary cells and primary cell lines.

The cells include T-cells, neutrophils, and several B-cell lymphomas. The conditions for electroporation were developed using Bio-Rad’s Gene Pulser MXcell™ electroporation system and Gene Pulser® electroporation buffer. The protocols can be viewed in Bio-Rad’s Gene Transfer Protocol library via the company article webpage link.

About Gene Transfer

Gene transfer is a general term used to denote transfection or transformation — the transfer of new genetic material into a cell. Bio-Rad offers several tools for gene transfer including electroporation, lipid-mediated delivery, and particle delivery. Electroporation is a valuable tool for nucleic acid delivery because it can be used for a wide variety of cell types, in particular, primary cells and cells that are difficult to transfect. Lipid-mediated delivery is widely used for mammalian cells. Particle delivery is useful for plant tissues