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60-Minute Listeria Test Advances Food Safety

Neogen Corp.

Neogen Corporation has developed an innovative test that detects Listeria in environmental samples in under an hour — without the need to enrich samples.

2 Aug 2017
Abigail Berry
Administrator / Office Personnel

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Neogen’s new Listeria Right Now™ test system can detect all species of Listeria, including the pathogenic L. monocytogenes, in under 60 minutes through their ribosomal RNA (rRNA). The system’s effectiveness has been validated by NSF International to detect low levels of Listeria in environmental samples with greater sensitivity and speed than any other method available. The test is also under review for AOAC Performance Tested certification to further validate its accuracy.

“The advanced technology in Listeria Right Now truly changes everything about testing the environment for Listeria. Contamination of Listeria in the environment can now be determined, and cleaned as necessary, before food production begins and the quality and safety of a food product is compromised,” said Ed Bradley, Neogen’s vice president of Food Safety. “The new test system enables food safety professionals to very quickly implement corrective actions, which could include improving sanitation efforts or processes. Using Listeria Right Now also means that companies no longer have to grow potentially dangerous cultures in their facilities during the testing process, nor store test cultures for potential follow-up testing.”

The innovative Listeria Right Now process starts with taking an environmental sample to capture any Listeria present. The entire swab sample is placed in a tube that contains a lysis buffer that breaks up any bacteria present, and releases its rRNA. If Listeria is in the sample, the test’s reagents will amplify thousands of copies of its rRNA — and make the Listeria easily detectable by Listeria Right Now.

In the validation study of Listeria Right Now comparing the test against the reference cultural method, NSF International confirmed the effectiveness of this new test. The evaluation determined that under the conditions employed in this study, the enrichment-free Listeria Right Now method is as sensitive as the enrichment-based culture reference method.

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MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Particle CharacterizationParticle characterization instruments are used to determine particle size distribution, shape, surface area, zeta potential, density and porosity of particles and materials. Multiple tecchniques are available for determining particle size, shape and count including dynamic light scattering (DLS), laser diffraction, electrozone (Coulter technique), imaging particle analysis and single particle optical sensing. Determine the density of your material with a gas pycnometer or examine its surface area and porosity with gas adsorption analyzers and mercury porosimeters. Find the best particle characterization instruments in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Food SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. Food samples can be tested using GC/MS, HPLC, SPE, and qPCR to ensure their adherence to regulations.RNARNA is a nucleic acid that plays a key role in gene expression and protein synthesis. It serves as a messenger between DNA and ribosomes, carrying genetic instructions to produce proteins. Advances in RNA-based therapies, such as mRNA vaccines and gene editing, have revolutionized treatment strategies for genetic disorders and infectious diseases. Explore RNA research tools and therapies in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.Pathogen DetectionPathogen detection is the process of identifying harmful microorganisms, including bacteria, viruses, and fungi, that can cause diseases. Accurate pathogen detection is critical for food safety, public health, and clinical diagnostics. Explore pathogen detection products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Environmental AnalysisEnvironmental analysis describes a variety of tests that determine the effect of chemicals, processes and particulates such as persistent organic pollutants (POPs) have on the environment.Listeria

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60-Minute Listeria Test Advances Food Safety