ResourceSeparations

Identification of NISTmAb Charge Variants by Fully Automated CIEF/MS

6 Aug 2020

This Application Note by Agilent demonstrates the direct, high-resolution, mass spectrometry (MS) identification of the NISTmAb charge variants using an online CIEF/MS workflow.

Agilent 7100 Capillary Electrophoresis System

Agilent Technologies

The Agilent 7100 CE System can be used as a standalone unit, as the separations component of a CE/MS, or as a complementary, orthogonal technology to LC. Extended light path capillaries increase the system’s sensitivity, while the replenishment system enables high-throughput operation.

(23)

Links

Tags

UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.
Identification of NISTmAb Charge Variants by Fully Automated CIEF/MS