Drastically improve the efficacy of DNA extraction from volcanic ash soil

3 Dec 2019

The challenge with extractions from soil is isolating DNA or RNA without contamination by humic acids or other PCR inhibitors. Volcanic ash soil, such as andisol, is known to be very difficult because this soil has a complex matrix. In this application note, learn how to improve DNA extraction.

FastDNA SPIN Kit for Soil™

MP Biomedicals

Isolate Pure DNA from Cells Present in Soil or Other Environmental Samples! Thorough lysis in seconds of any microorganism present in environmental samples. Ready-to-use DNA for quantitative and qualitative characterization of microbial soil communities. Excellent reproducibility for an optimum assay-to-assay consistency. Total removal of humic acids and PCR inhibitors for a successful investigation of microbial diversity. The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungal, plant and animal genomic DNA from soil and environmental samples. Up to 500 mg soil are processed by the FastPrep®-24 5G or and any bead beating homogenizers with the Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive bacteria and yeast. The released DNA is purified by a silica-based spin filter method and is suitable for PCR and other downstream applications.    

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Drastically improve the efficacy of DNA extraction from volcanic ash soil