Application Note: CLARIOstar Plus Simplifies Enzymatic Reaction Monitoring with its Novel Enhanced Dynamic Range Technology
22 February 2019

Here, the detection of a β-gal enzyme assay that is primarily used for reporter assays was demonstrated. With the help of the EDR technology of the CLARIOstar Plus, β-gal mediated production of fluorescein was conveniently monitored. It allowed the detection of huge signal differences covering a range from 660 RFU up to 3.44*108 RFU. Translated to a reporter assay, this means very high and very low expressions can be measured at the very same plate. The tool simplifies the set-up of enzymatic assays as it does not require time to think about the right gain settings or repeat the assay until optimal settings are found.