A Simplified Protocol to Streamline Inhibitor Profiling with the Transcreener® ADP2 FP Assay
13 Sept 2015Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes - many of which catalyze the covalent regulatory reactions that are central to cell signaling and are of great value as targets in drug discovery. The Transcreener® ADP2 FP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of ADP and is a simple, one-step homogenous detection assay. This Application Note describes how to streamline the process of inhibitor profiling with the Transcreener® ADP2 FP Assay eliminating the need to experimentally optimize the ADP2 antibody concentration and the need for a standard curve.
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Transcreener® ADP² Kinase Assays
BellBrook LabsThe Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.