Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit
24 Feb 2021Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. However for biological applications involving small samples, such as next-generation sequencing and quantitation of DNA amplification products, sensitive methods are needed.
In this application note, Molecular Devices demonstrates how the Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA.
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