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Resolution of adeno-associated viral vector aggregates and fragments with Agilent Bio SEC-5
31 Jan 2022Adeno-associated viruses (AAVs) are a promising new class of biotherapeutic with many proven applications. This application note demonstrates the chromatographic resolution of AAV aggregates and fragments using an Agilent Bio SEC-5 column.
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Agilent Bio SEC-5
Agilent TechnologiesAgilent Bio SEC-5 HPLC columns are packed with 5 µm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution.
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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.CentrifugesCentrifuges are used to separate particulates suspended in a liquid via the application of a centrifugal force including cells, macromolecules, nanoparticles and precipitates. Centrifuges come in three sizes: microcentrifuges, benchtop and floor-standing centrifuges, depending on the volume and number of samples that require separation. When choosing a centrifuge, consider its maximum separation power indicated by its relative centrifugal force (RCF) value. Low force centrifuges reach a maximum RCF of 8,000 g, high-force centrifuges can reach maximum RCF values of 80,000 g. For extremely high forces, consider ultracentrifuges (up to 800,000 g). Refrigerated and vacuum centrifuges also known as concentrators are available for more specialized applications. A range of volumes are available for centrifuge tubes and microfuge tubes, depending on sample sizes. Find the best centrifuges in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.VirusesViruses are microscopic pathogens that require a host cell to replicate. Understanding their structure, replication cycle, and impact on the immune system is crucial in developing effective treatments, vaccines, and diagnostic methods. Research continues to focus on emerging viral diseases and antiviral drug discovery. Explore solutions for virus research, detection, and treatment in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.SECSize exclusion chromatography (SEC) separates molecules based on size, ideal for protein purification, polymer analysis, and biopharma applications. SEC ensures precise characterization of biomolecules for research and industry. Explore peer-reviewed SEC products and compare options for your lab.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.