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Optimizing analysis and purification of a synthetic peptide using PLRP-S columns

24 May 2023

Reversed-phase ion-pair chromatography has become increasingly useful for the analysis and purification of synthetic peptides using mobile phases containing trifluoroacetic acid (TFA) as an ion-pair reagent. This application note from Agilent describes the analysis of a synthetic peptide and how to scale-up methods using an Agilent PLRP-S analytical HPLC column.

PLRP-S for Biomolecules

Agilent Technologies

PLRP-S for Biomolecules products are available in a range of pore sizes and particle sizes, all with identical chemistry and fundamental adsorptive characteristics. The particles are inherently hydrophobic so there is no bonded phase, alkyl ligand required for reversed-phase separations.

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