Oligo Pools: Design. Build. Test.

Twist Oligo Pools are highly diverse collections of single-stranded oligo-nucleotides synthesized using our silicon-based DNA writing technology. Our synthesis platform enables massively parallel production of hundreds of thousands of high-quality, accurate oligos per run. Oligo pools are utilized in many applications including generation of CRISPR guide RNA (sgRNA) libraries, peptide and protein screens, and high-throughput reporter assays.