Interrogating PRMT5, MLL4, METTL3/14, and NSP14 with the AptaFluor SAH Assay

30 October 2023

In this application note from BellBrook Labs, explore how the Aptafluor^® SAH Assay provides a reliable and robust tool for detecting activity of four methyltransferases (MTs) using various acceptor substrates, including peptides, DNA and RNA. The AptaFluor SAH Methyltransferase Assay is a high-throughput enzymatic assay for SAM-dependent methyltransferases. The assay relies on a naturally occurring SAH-sensing RNA aptamer, or riboswitch, that binds SAH with nanomolar affinity and exquisite selectivity.