Direct and rapid multi-attribute monitoring of intact monoclonal antibodies by icIEF-UV/MS

26 January 2024

In this application note, SCIEX introduces a novel integrated workflow utilizing the Intabio ZT system. The system integrates imaged capillary isoelectric focusing (icIEF) with mass spectrometry (MS), providing a robust method for characterizing proteins and quantifying charge variants. The application focuses on the critical need for characterizing charge heterogeneity in recombinant monoclonal antibodies (mAbs), highlighting the potential impact of post-translational modifications during the manufacturing process.