Cell line engineering with CRISPR-Cas9: Validation strategies

Once you've identified your clones from your CRISPR screen, the next stage is to validate them. Sarah Dickerson, Ph.D, R&D Scientist at Horizon Discovery discusses how you should go about validating your clones of interest, the options for functional assays and what to do if an antibody based functional assay isn't available for your edit of interest as well as how to go about analyzing off-target editing events.