Emily Hardy, Manager of Cell Engineering Services at Horizon Discovery, discusses cell line optiization techniques including what reagent formats yo...
Generating a knock-in cell line adds another line of complexity to the relatively straightforward knockout application. Guide RNA can be more tricky...
Kevin Hemphill, R&D Scientist at Horizon Discovery, discusses the genetic considerations you should think about prior to embarking on generating a c...
Once you've run your CRISPR experiment and you have a pool of mixed genotypes, finding clones with the genotypes of interest and determining how man...
Steve Lenger, Ph.D., bioinformatic expert at Horizon Discovery, shares his insights into what makes a good guide RNA when conducting knockout expeer...
Jennifer Abarca, Head of Scientific Support for Horizon Discovery, gives a brief overview of the CRISPR system, what pathways are used to generate k...
Once you've identified your clones from your CRISPR screen, the next stage is to validate them. Sarah Dickerson, Ph.D, R&D Scientist at Horizon Disc...