Size and fluorescence calibrated imaging flow cytometry: From arbitrary to standardized units

Thursday, November 20, at 15:00 GMT | 16:00 CET | 10:00 EST | 7:00 PST

Imaging flow cytometry (IFC) has emerged as a powerful approach for single extracellular vesicle (EV) characterization, yet its broader adoption has been hindered by challenges in assay standardization and data comparability. This webinar will highlight recent methodological advances addressing these limitations. Building on earlier work that established workflows for direct detection and phenotyping of EVs in unprocessed plasma, current efforts focus on developing rigorous calibration strategies for IFC.

Recent work has introduced bead-based size calibration and fluorescence intensity standardization, enabling instrument-specific signals to be expressed in quantitative units. These approaches markedly improve reproducibility across different platforms and laboratories, thereby supporting cross-study comparability. Standardized IFC data further provide a framework for quantitative analyses in both fundamental EV research and the quality assessment of vesicle-based therapeutics.

Together, these methodological advances position IFC as a calibrated, quantitative platform for nanoparticle cytometry, with the potential to accelerate both biomarker discovery and translational applications in extracellular vesicle science.

Topics discussed in this webinar will include:

• Advances in EV assay standardization and data comparability
• Development of calibration strategies for IFC
• Introduction of bead-based standardization methodology for quantitative EV analyses

Who should attend?

• Researchers using IFC who are currently or wish to perform small particle analysis
• Anyone interested in flow cytometry applications and small particle analysis

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

For Research Use Only. Not intended for use in diagnostic procedures.