New Resin for the Purification of His-Tagged Proteins
23 Apr 2013
Watch this video to learn how the new cOmplete His-Tag Purification Resin from Roche Applied Science enables His-tagged proteins to be purified in more favorable conditions. Steve Bye explains how the Roche’s new proprietary nickel-chelate chemistry is compatible with DTT and EDTA. This means scientist’s can now maintain the highest protein stability during purification, without compromising the capacity of the column. Interview filmed by SelectScience at AACR 2013.
About the company
Roche
We believe it’s urgent to deliver medical solutions right now – even as we develop innovations for the future. We are passionate about transforming patients’ lives. We are courageous in both decision and action. And we believe that good business means a better world. That is why we come to work each day. We commit ourselves to scientific rigour, unassailable ethics, and access to medical innovations for all. We do this today to build a better tomorrow. We are proud of who we are, what we do, and how we do it. We are many, working as one across functions, across companies, and across the world. We are Roche.
Related products
Request Quote for All Products
cOmplete His-Tag Purification Resin
cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC (Immobilized Metal Affinity Chromatography) matrix for convenient single-step purification of His-tagged proteins from lysates. Roche's new proprietary nickel-chelate chemistry is compatible with commonly used reducing agents (such as DTT), chelating metalloprotease inhibitors (such as EDTA), and a wide range of buffer substances and salt conditions. The wide choice of compatible ingredients makes it easy to optimize buffers for high protein stability and solubility and to protect your proteins from proteases and oxidation. Stabilize your protein with DTT and EDTA. Use any buffer with EDTA or DTT for the highest protein stability without loss of capacity. Produce large amounts of highly purified proteins. High binding capacity and specificity are ideal for one-step purifications. Generate less toxic waste. Negligible nickel leakage reduces toxic waste and avoids nickel recharging.
