Mouse on mouse labeling with Fab fragment blocking
5 Oct 2020
This video by Jackson ImmunoResearch Laboratories demonstrates how to use Fab fragments to successfully label primary antibodies from the same species as the tissue sample (e.g. mouse on mouse).
About the company
Jackson ImmunoResearch Laboratories Inc.
Jackson ImmunoResearch Laboratories, Inc. specializes in the production and conjugation of affinity-purified secondary antibodies and purified immunoglobulins. Its products are sold primarily to scientists in universities and research institutes throughout the world who are conducting research in the plant, animal, and biomedical sciences.
The company is located in rural Pennsylvania, near the Amish country, about 40 miles west of Philadelphia. It was started in 1982 by scientists whose education and previous experience included research in cell biology, protein chemistry, microbiology, and product development in immunology. The company’s goal is to provide other scientists with the newest, largest selection of, and highest quality of secondary reagents, with the best technical and customer services possible. The products are for research use only, and not for diagnostics or therapeutics. They are not primary antibodies, nor are they medical devices.
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F(ab')2 Fragment Affinity-Purified Secondary Antibodies
Jackson ImmunoResearch Laboratories Inc.F(ab')2 fragments of antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving intact some of the hinge region. Used for specific applications, such as to avoid binding of antibodies to live cells with Fc receptors or to Protein A or Protein G.
Fab Fragment Affinity-Purified Secondary Antibodies
Jackson ImmunoResearch Laboratories Inc.Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. Block endogenous immunoglobulins on cells, tissues , or other surfaces. Block exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species. Fab-label primary antibodies in solution without compromising activity.
