Rapid peptide mapping and monitoring of post translational modifications using high resolution ion mobility mass spectrometry
18 June 2021

This application note describes a HRIM-LC-MS method to baseline resolve isobaric PTMs in a mAb tryptic digest, enabling a 4.5X reduction in analysis time from traditional peptide mapping workflows. Incorporating HRIM into an LC-MS workflow provided >96.5% sequence coverage in just 20 minutes total analysis time and included critical structural information that would have been missed using conventional LC-MS techniques alone.