A simple and straightforward procedure for measuring functional kinase activity.The Eu(K) labeled antibody and XL665 conjugate are added simultaneously after the enzymatic step. Results are available after a 2-hour incubation at room temperature. The two reagents are stable in solution, even in an excess of EDTA (0.5M), while the assay remains stable for >24 hours. An extended list of Eu3+-Cryptate labeled phospho-specific…
CRISPR/CAS9 Gene Editing of Human Induced Pluripotent Stem Cells (iPSCs)
Streamline gene activation experiments with stably expressing dCas9-VPR cell lines
CompoZr ® ZFN (zinc finger nuclease) technology was used to insert the sequence for fluorescent proteins adjacent to endogenous genes to create reporter cell lines for compound screening
This pooled library targets the human kinome, a collection of approximately 700 genes commonly associated with development and disease, with an average of nearly 9 unique knockout CRISPR clones per kinase gene. The lentiviral format allows for stable integration, selection, and enrichment of transduced cells
Components of the Upstate (Millipore) Casein Kinase 2 Assay Kit:• Casein Kinase 2 Substrate Peptide (Cat.# 12-330) • Assay Dilution Buffer I (ADBI) (Cat.# 20-108) • PKA Inhibitor Cocktail (Cat.# 20-114) • Magnesium/ATP Cocktail (Cat.# 20-113) • P81 Phosphocellulose Squares (Cat.# 20-134) For use to measure the phosphotransferase activity of Casein Kinase 2 (CK-2) in immunoprecipitates and column fractions based on phosphorylat…
Ambion® Cells-to-CT™ Kits allow you to measure relative gene expression by real-time RT-PCR without having to purify RNA prior to amplification. Cells-to-CT™ Kits are available for both TaqMan® and SYBR® Green detection. Cells-to-CT™ kits provide a complete workflow for real-time qRT-PCR analysis directly from cultured cells without RNA purification. Featuring a unique method for lysing cultured cells while removing genomic…
Unlike other suppliers of QuEChERS extraction kits, Agilent QuEChERs salts and buffers are pre-packaged in anhydrous packages. This allows you to add them after adding your solvent to the sample, as specified in the QuEChERS methodologies. Extraction Kits include: 50 quantity of 50 mL polypropylene centrifuge tubes with caps MgSO4 and NaCl, or other salts for buffering; pre-weighed in anhydrous packet
Guest editorial article by Vikas Singh, PhD, and Thomas Beadnell, PhD, from Eurofins
Hear how DNA analysis helped unravel the mystery
Engage with leading minds and the latest technology solutions to tackle today’s analytical challenges
A Bead Bath performs all the essentials, including warming, thawing, incubating, chilling and does it while maintaining an constant temperature just like a water bath. It is known that MICROBES thrive in water, yet water is ever-present in laboratories, healthcare facilities, and production and manufacturing clean-room facilities. Water and ice enter the workspace through various distribution systems and are a component of m…
Agilent CrossLab is a growing portfolio of GC supplies manufactured to perform seamlessly with the non-Agilent GCs in your lab. From autosampler syringes to autosampler vials, Ultra Inert inlet liners to non-stick liner O-rings, capillary column ferrules to column nuts, vials, caps, septa, and more. Experience the same Agilent quality solutions you know and trust, now for your non-Agilent GCs: PerkinElmer GC Systems…
Gibco TrypLE reagents are highly purified, recombinant cell-dissociation enzymes that replace porcine trypsin. These reagents are ideal for dissociating attachment-dependent cell lines in both serum-containing and serum-free conditions, and can be directly substituted for trypsin without protocol changes.
HR spectrometers are small-footprint, high-resolution spectrometers for applications such as wavelength characterization of lasers and LEDs, monitoring of gases and monochromatic light sources, and determination of elemental atomic emission lines. Depending on spectrometer configuration, optical resolution <0.1 nm (FWHM) is possible.
EVE HT is a high-throughput automated multiple cell counter, providing 48-sample counting in just 2.5 minutes for primary cells and cell lines analyses. With small sample volume and extensive capacity, the EVE HT provides accurate cell counting and viability measurements.
Corning® Collagen I, rat tail, 1g (10 x 100mg), is used either as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function. Source Rat Tail Tendon Quality Purity = 90% by SDS-PAGE Tested for ability to promote attachment and spreading of HT-1080 human fibrosarcoma cells Tested for formation of a firm gel up to a dilu…
Amine-reactive TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry.
High-profile appointment brings valuable experience and insight as SelectScience expands clinical offering and focus
