Search results for "kings college london"

Chemicon's Y2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of Y2 interactions and its ligands. The membrane preparations exhibit a Kd of 1.28 nM for [125I]-human PYY. With 0.5 nM [125I]-human PYY, 5 or 10µg/well Y2 Membrane Prep yields greater than 10-fold…

Chemicon's VPAC2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of VPAC2 interactions with PACAP. The membrane preparations exhibit a Kd of 0.9 nM for [125I]-PACAP27. With 5 µg/well VPAC2 Membrane Prep and 0.5 nM [125I]-PACAP27, a greater than 15-fold signal…

Chemicon's α1A membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of α1A interactions with prazosin. The membrane preparations exhibit a Kd of 1.34 nM for [3H]-prazosin. With 5 µg/well α1A Membrane Prep and 1 nM [3H]-prazosin, a greater than 7-fold signal-to-bac…

Chemicon's membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of κ interactions with diprenorphine. The membrane preparations exhibit a Kd of 2.3-3.7 nM for [3H]-diprenorphine. With 10 µg/well κ Membrane Prep and 2.5 nM [3H]-diprenorphine, a greater than 3-fold…

Chemicon's D3 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of D3 interactions and its ligands. The membrane preparations exhibit a Kd of 0.1 nM for [125I]-Iodospiperone. With 5.0ug/well D3 membrane prep and 0.1 nM [125I]-Iodospiperone, a greater than 4-fol…

EMD Millipore's 5-HT1B membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of 5-HT1B. The membrane preparations exhibit a Kd of 10.5 nM for [3H]-GR125743. With 7 nM [3H]-GR125743, 10μg/well 5-HT1B Membrane Prep typically yields greater than 8-fold si…

EMD Millipore's GIP membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at GIP Receptor. The membrane preparations exhibit a Kd of 0.44 nM for [125I]-GIP. With 0.2 nM [125I]-GIP, 10 µg/well GIP Receptor Membrane Prep typically yields greater than 8-f…

Chemicon's CXCR7 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at CXCR7. The membrane preparations exhibit a Kd of 0.32 nM for [125I]-SDF-1α. With 0.25 nM [125I]-SDF-1α, 10µg/well CXCR7 Membrane Prep typically yields greater than 4-fold signal-…

EMD Millipore's S1P1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of S1P1 interaction with its ligands. The cell line exhibits a calcium response with EC50s of 141 μM for Sphingosine 1-phosphate. The membrane preparations exhibit EC50s of 9.5 μM for Sphingosine 1-phospha…

The SatisFection reagent is a novel cationic polymer suitable for both transient and stable transfections in the presence or absence of serum. This polymer functions by binding and condensing DNA into a polyplex that enters the cell via endocytosis.  Cost savings of up to 70% per transfection  Unmatched performance in some hard-to-transfect lines  Maximum expression 36 - 72 hours post-transfection  Ideal reagent for exp…

The SatisFection reagent is a novel cationic polymer suitable for both transient and stable transfections in the presence or absence of serum. This polymer functions by binding and condensing DNA into a polyplex that enters the cell via endocytosis.  Cost savings of up to 70% per transfection  Unmatched performance in some hard-to-transfect lines  Maximum expression 36 - 72 hours post-transfection  Ideal reagent for exp…

No logo or lettering for improved heat sealing and ease of liquid handlingRaised well bottom for higher sensitivityExcellent signal dynamic range due to low background fluorescence and enhanced signal intensitySuperior flatness -minimizes variability within a plate and between platesFlat bottom wells are compatible with automated dispensers including the PerkinElmer FlexDrop™Not treated (or medium binding) polystyrene surface…

CoolCell® alcohol-free cell freezing containers ensure standardized controlled rate -1? /minute cell freezing in a -80? freezer — without alcohol or any fluids. Proven for use with a variety of cell types including stem cells, primary cells, PBMC cell lines, insect cells, yeast and others. The patent-pending CoolCell technology utilizes a thermo-conductive alloy core and highly-insulative outer material to control the rate of…

Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interactions Is suitable primarily for the immobilization of large molecules, such as antibodies, that have large hydrophobic regions that can interact with the surface Untreated microplates have a binding capacity of approximately 100 to 200ng IgG/cm2 Without lids, lids supplied separately Recommended work…

Cisbio Bioassays has developed Cellul'erk, a highly accurate HTRF assay for measuring phosphorylated ERK1/2 in 96-, 384- and 1536-well formats providing for an HTS compatible assay. Features: • Simple assay protocols designed for research studies (96-well plates) for HTS (384-small vol. plates) • Cell-based functional assay • Antibody-based detection • Direct detection Applications: • Gi, Gs, or Gq coupled receptor activatio…

Measure elemental, ionic and total mercury in exhaust stacks from coal-fired boilers and waste incinerators with the Thermo Scientific™ Mercury Freedom System. Meet or exceed performance specifications outlined in U.S. EPA PS-12A and/or Part 75 provisions for continuous Mercury CEM systems in addition to the latest MATS and MACT rules.Ensure complete regulatory compliance with unattended, true continuous monitoring. The Therm…

Agilent’s Quick Amp Labeling Kits reliably amplify and label target RNA for the robust generation of cRNA. Sample input of 200 ng total RNA Multiple kit formats available with one- or two-color labeling protocols Includes reagents for 20 reactions   For Research Use Only. Not for Use in Diagnostic Procedures.

The MagNA Pure Compact Tip Tray Kit is required for performing the Leakage Test on the MagNA Pure Compact Instrument, and is used as replacement for lost or contaminated Tip Trays from the MagNA Pure Compact Isolation Kits. One Tip Tray contains all the required disposables for one nucleic acid extraction.

The Bead Ruptor 12 is the most powerful and economical bead mill homogenizer available today. It is an ideal solution for a variety of applications that require grinding, lysing, and homogenization of biological samples prior to molecular extraction. Using disposable sample tubes pre-filled with a variety of lysing beads, the Bead Ruptor 12 vigorously and uniformly shakes up to 16 tubes at a time providing an efficient, con…

Millipore’s FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis is designed to allow the researcher to easily identify, discriminate, and quantitate cells between the G2 and M-phases of the cell cycle. The kit can be used to perform bivariate analysis to measure cell cycle distributions by discrimination of cell cycle phases without the need of sophisticated software modules or algorithms. Having the ability to distingui…

KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis.  KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent rea…

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors. Source Engelbreth-Holm-Swarm Mouse Tumor Quality Mouse colonies routinely screened for pathogens via Mous…

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.Quality Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing Extensive…

Bottom surface options: 0.72 or 0.17mm glass (custom available on request) and clear quartz. Glass bottom plates are scratch resistant. Glass bottom plates are microbubble resistant Black polystyrene upper microplate structure. Applications include fluorescence assays such as laser-induced fluorescence (LIF), Fluorescence Correlation Spectroscopy (FCS); and Nephelometry. Ideal for cell-based assays using Scanning and Confocal…

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix (ECM) proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.Quality Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing Extensive…